Fig. 1

Timeline of the behavioral experiment. Seven days after arrival, mice were received viral delivery of channelrhodopsin. The fiber optic cannulae were placed on day 21. Behavioral testing began on approximately day 28 with the baseline test, followed by 10 Hz acquisition then reversal trials. 20 Hz acquisition and reversal trials, then 30 Hz acquisition and reversal trials followed spaced approximately one week apart, and for females, in accordance with the appropriate phases of the estrous cycle. Mice were perfused following the final behavioral trial