Fig. 2
Quantification of neurite formation in primary cortical cells isolated from the prefrontal cortex of the rat offspring prenatally exposed to BPA or vehicle control. (a) Cortical cells were isolated from neonatal rat pups prenatally exposed to BPA or the vehicle control and cultured for 14 days. Images of the cells were taken using an inverted microscope. (b, c) Total neurite length (treatment F1,34 = 0.097 and P value = 0.756, sex F1,34 = 24.639 and P value < 0.001, P value < 0.001 for treatment × sex, by two-way ANOVA), (d, e) average branch length (treatment F1,34 = 2.325 and P value = 0.128, sex F1,34 = 0.011 and P value = 0.918, P value = 0.021 for treatment × sex, by two-way ANOVA), (f, g) number of branches per neuron (treatment F1,34 = 1.070 and P value = 0.302, sex F1,34 = 33.772 and P value < 0.001, P value < 0.001 for treatment × sex, by two-way ANOVA), (h, i) primary neurite length (treatment F1,34 = 1.889 and P value = 0.170, sex F1,34 = 6.667 and P value = 0.010, P value < 0.001 for treatment × sex, by two-way ANOVA), (j, k) number of primary neurites per neuron (treatment F1,34 = 0.744 and P value = 0.389, sex F1,34 = 1.951 and P value = 0.163, P value < 0.001 for treatment × sex, by two-way ANOVA), and (l, m, n) Sholl analysis of primary cortical neurite (treatment F1,34 = 14.303 and P value < 0.001, sex F1,34 = 38.658 and P value < 0.001, radius F1,34 = 780.439 and P value < 0.001, P value < 0.001 for treatment × sex × radius, by three-way ANOVA) were determined. Data are presented as the mean ± SEM. *P value < 0.05