Fig. 4
Prenatal exposure to BPA did not affect sociability evaluated by the three-chamber test. (a) The sociability and social novelty in the offspring were assessed using three-chamber tests. (b, c) For the sociability test, the total times that the rat spent in the empty chamber and chamber containing a stranger rat (treatment F1,18 = 4.546 and P value = 0.047, sex F1,18 = 0.014 and P value = 0.906, P value = 0.342 for treatment × sex, by two-way ANOVA), (d, e) the time that the rat spent in the empty chamber or chamber containing a stranger (treatment F1,68 = 1.808 and P value = 0.187, sex F1,68 = 0.006 and P value = 0.940, chamber F1,68 = 107.885 and P value < 0.001 and P value = 0.726 for treatment × sex × intruder, by three-way ANOVA), (f, g) preference index of time spent in the empty or stranger chamber were measured (treatment F1,18 = 1.515 and P value = 0.234, sex F1,18 = 0.464 and P value = 0.505, P value = 0.920 for treatment × sex, by two-way ANOVA), and (h, i) the times that the rat spent interacting (sniffing time) with a stranger (treatment F1,18 = 0.904 and P value = 0.354, sex F1,18 = 0.552 and P value = 0.467, P value = 0.101 for treatment × sex, by two-way ANOVA). Data are presented as the mean ± SEM. *P value < 0.05