Fig. 5
Prenatal exposure to BPA impaired social novelty evaluated by the three-chamber test in a sex-dependent manner. (a) The total times that the rat spent in the familiar and novel chamber (treatment F1,18 = 0.183 and P value = 0.674, sex F1,18 = 0.336 and P value = 0.570, P value = 0.993 for treatment × sex, by two-way ANOVA), (b) total time that the rat spent interacting with familiar and novel rat (treatment F1,18 = 0.648 and P value = 0.431, sex F1,18 = 0.038 and P value = 0.849, P value = 0.040 for treatment × sex, by two-way ANOVA), (c) the time that the rat spent in the familiar chamber or novel chamber (treatment F1,68 = 0.069 and P value = 0.795, sex F1,68 = 0.126 and P value = 0.724, chamber F1,68 = 45.260 and P value < 0.001 and P value = 0.182 for treatment × sex × intruder, by three-way ANOVA), (d) The time that the rat spent interacting with familiar or novel rat (treatment F1,68 = 0.797 and P value = 0.378, sex F1,68 = 0.046 and P value = 0.831, chamber F1,68 = 22.845 and P value < 0.001 and P value = 0.328 for treatment × sex × intruder, by three-way ANOVA), (e) the preference index of time spent in the familiar or novel chamber (treatment F1,18 = 6.494 and P value = 0.020, sex F1,18 = 2.022 and P value = 0.172, P value = 0.219 for treatment × sex, by two-way ANOVA), and (f) the preference index of time that the rat spent interacting with familiar and novel rat were measured (treatment F1,18 = 1.876 and P value = 0.188, sex F1,18 = 0.030 and P value = 0.863, P value = 0.232 for treatment × sex, by two-way ANOVA). Data are presented as the mean ± SEM. *P value < 0.05