Fig. 4
From: Sex- and species-specific contribution of CD99 to T cell costimulation during multiple sclerosis
Cd99-deficient mice show no difference in immune cell function. A CD99 surface expression of immune cells in C57BL/6J WT mice (n = 5 females, n = 5 males). B Representative flow cytometry plots of CD99 surface expression on immune cells shown in (A). C CD99 surface expression of T cells in homozygous Cd99–/–, heterozygous Cd99–/– and WT littermates (n = 4 female d/d, n = 2 male d/d, n = 3 female + /d, n = 3 male + /d, n = 3 female +/+, n = 3 male +/+). D Cell proliferation assay with isolated T cells from homozygous Cd99–/–, heterozygous Cd99–/– and WT littermates (n = 2 females and 1 male per group). E EAE course in homozygous Cd99–/–, heterozygous Cd99–/– and WT littermates after active immunization against MOG35-55 peptide (n = 8 female d/d, n = 7 male d/d, n = 6 female + /d, n = 7 male + /d, n = 8 female +/+, n = 7 male + / +). Data are shown as mean ± SEM. Statistics: A two-way ANOVA with Sidak’s post-hoc; C two-way ANOVA with Tukey post-hoc; D Kruskal–Wallis test with Dunn post-hoc; E Mann–Whitney U test; *P < 0.05; **P < 0.01; ***P < 0.001