Fig. 2

Effect of miR-21 on DHT-mediated changes in energy homeostasis, locomotion and sleep. WT or miR21KO mice were treated with dihydrotestosterone (DHT) or vehicle (Veh) for 90 days. A, B Energy expenditure (EE) was determined by indirect calorimetry (N = 6–7/group). C Food intake is expressed as Kcal and normalized by body weight (N = 6–8/group). D Sleep time, defined as the animal's immobility for more than 40 s, was measured in hr (N = 7–10/group). E, F Locomotor activity (E) was determined based on the number of infrared light beams breaks mounted in the cages in X, Y, and Z axes, and the total distance the animals moved in the cage (F) was measured in meters (N = 5–8/group). G, H Oxygen consumption (VO2) (G) and carbon dioxide production (VCO2) (H) was measured as the volume of gas produced/consumed/hr and normalized by body weight (N = 6–7/group). I, J The respiratory exchange ratio (RER) was calculated as the VCO2/VO2 ratio (N = 6–7/group). Data are expressed as mean ± SEM. Data were analyzed by two-way ANOVA followed by Fisher’s LSD test. *p < 0.05; **p < 0.01; ***p < 0.001