Fig. 7

PCR and Sanger sequencing validated the 70 bp deletion of the WWOX gene. (A) Gel electrophoresis of PCR products derived from normal female pig exclusively displayed wild-type bands (referred to as band A, 298 bp). Conversely, in XX DSD pigs, discernible deletion bands surfaced, encompassing homozygous (referred to as band B, 228 bp) as well as heterozygous (228 bp and 298 bp) deletions. (B) The sanger sequencing results showed a 70 bp deletion between the wild type and homozygous type. The sequencing results of the heterozygous bands were manually corrected to show two strands with a 70 bp difference. Black boxes indicate the 70 bp deletion. WT indicates wild-type, Het indicates heterozygous, and Hom indicates homozygous