Fig. 1

Timeline of adolescent social isolation procedures. EASI (PD 21–42) and LASI (PD42-63) rats underwent three weeks of social isolation and were then rehoused with rats from the same condition, whereas control rats remained group-housed throughout the study. In the first experiment, the rats underwent behavioral testing starting at PD 90. In the second experiment, rats were sacrificed on PD 90 for comparison. The timeline was created with BioRender.com