Fig. 3

Maternal adipose-specific Ghr deficiency increases body fat mass and adipocyte size in female offspring fed regular chow. A: Body weights of 3-week-old female offspring of KO and LL maternal mice (n = 5–6 mice/group). B: Growth curves of female offspring of KO and LL maternal mice during RC feeding (n = 4–5 mice/group). C: Fat tissue weight of female offspring of KO and LL maternal mice under RC feeding (n = 4–5 mice/group). D: H&E staining of WAT sections. Scale bar: 100 μm. E and F: Quantification of the adipocyte area in sWAT (F) and gWAT (G). The average area of adipocytes was analyzed using ImageJ software on H&E-stained slides from at least three fields of three mice per genotype. All the values are presented as the means ± SEMs. The statistical significance was indicated by *p < 0.05, **p < 0.01 and ***p < 0.001.