Fig. 2
Higher DRD1 and DRD3 expression in B cells from men was partly regulated by estradiol and testosterone. A, B Basal expression of DRD1, DRD2, DRD3, and DRD4 on monocytes (A) and B cells (B) from women and men measured via flow cytometry; n = 17–19 per group. C, D DRD1 expression on B cells from women and men after 24 h stimulation with E2 (10–8, 10–9, 10–10 M, C) and DHT (10–7, 10–8, 10–9 M, D) in mixed PBMC culture measured via flow cytometry and normalized to DMSO control; n = 8 per condition. E, F DRD3 expression on B cells from women and men after 24 h stimulation with E2 (10–8, 10–9, 10–10 M, E) and DHT (10–7, 10–8, 10–9 M, F) in mixed PBMC culture measured via flow cytometry and normalized to DMSO control; n = 8 per condition. G, H Correlation of DRD1 expression on B cells from women and men with basal estrogen (G) and testosterone (H) levels in plasma; n = 17–18 per group. I, J Correlation of DRD3 expression on B cells from women and men with basal estrogen (I) and testosterone (J) levels in plasma; n = 17–18 per group. Women: orange; men: grey. Unpaired t-test was used for comparing women and men, while simple linear regression was used to analyze the correlation of DR expression with sex hormone levels. One-way ANOVA with Geisser-Greenhouse correction and Dunnett multiple comparisons test was used for statistical testing of sex hormone receptor (SHR) stimulation with three concentrations of E2 and DHT. Mann–Whitney test compared the effects of SHR stimulation between women and men at the same hormone concentrations; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001