Fig. 4

Sex-specific cytokine secretion of monocytes after DR stimulation is dependent of B cells. A Representative flow cytometry plots of mixed PBMCs and CD19⁺ B cell-depleted PBMCs. B, C IL8 levels in supernatant from PBMCs and B cell-depleted PBMCs from women (B) and men (C) after 24 h in culture, with and without stimulation by A68930 (A, 10^–7 M) or Ropinirole (R, 10^–6 M) measured via ELISA; normalized to unstimulated control; n = 8–9 per group. D, E MCP1 levels in supernatant from PBMCs and B cell-depleted PBMCs from women (D) and men (E) after 24 h in culture, with and without stimulation by A68930 (A, 10^–7 M) or Ropinirole (R, 10^–6 M) measured via ELISA; normalized to unstimulated control; n = 8 per group. F, G Diagrams illustrating the interplay between B cells and monocytes after DR stimulation, showing effects on activation markers and cytokine secretion for cells from women (F) and men (G); created in BioRender.com. Wilcoxon test was used for paired data comparisons including PBMCs vs. B cells depleted as well as unstimulated vs. stimulated samples. Mann–Whitney test was used for testing statistical significance between unpaired data of women and men; *p ≤ 0.05