Fig. 5

Inflammatory condition turned DR stimulation-induced characteristics of female monocytes into proinflammatory phenotype. A Percentage of MCP1⁺ B cells, monocytes, T cells, and NK cells after 24 h in mixed PBMC culture, with or without CpG (0.195 μM) stimulation, measured via flow cytometry; n = 12 per condition. B MCP1 levels in supernatant from mixed and monocyte-depleted PBMCs after 24 h of CpG stimulation measured via ELISA; n = 14 per condition. C MCP1 levels in supernatant from PBMCs of women and men after 24 h in culture with CpG (0.195 μM) with or without A68930 (A, 10^–7 M) or Ropinirole (R, 10^–6 M) measured via ELISA; normalized to CpG control; n = 11–13 per group. Basal levels of unstimulated and CpG stimulated samples are presented in Supplementary Fig. 5C. D-G Percentage of CD69⁺ monocytes (D) and expression of HLA-DR (E), CD86 (F) and CD38 (G) on monocytes from women and men after 24 h in culture of PBMCs with or without CpG (0.195 μM) stimulation and after stimulation with CpG + A68930 (A, 10^–7 M) and CpG + Ropinirole (R, 10^–6 M) measured via flow cytometry; normalized to CpG control; n = 14 per group. Basal levels of unstimulated and CpG stimulated samples are presented in Supplementary Fig. 5D-G. Wilcoxon test was used for paired data comparisons including samples of two different stimulations as well as PBMCs vs. monocytes depleted. Mann–Whitney test was used for testing statistical significance between unpaired data of women and men; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001