Fig. 6

Proinflammatory phenotype of monocytes from women after DR stimulation is independent of B cells under inflammatory condition. A, B MCP1 levels in supernatant from mixed and B cell-depleted PBMCs from women (A) and men (B) after stimulation with CpG (0.195 μM), CpG + A68930 (A, 10^–7 M) or CpG + Ropinirole (R, 10^–6 M) measured via ELISA; normalized to CpG control; n = 7–8 per group. C, D Percentage of CD69⁺ monocytes from women (C) and men (D) after stimulation with CpG (0.195 μM), CpG + A68930 (A, 10^–7 M) or CpG + Ropinirole (R, 10^–6 M) measured via flow cytometry; normalized to CpG control; n = 7 per group. E–J HLA-DR (E, F), CD86 (G, H), and CD38 (I, J) expression on monocytes from women (E, G, I) and men (F, H, J) after stimulation with CpG (0.195 μM), CpG + A68930 (A, 10^–7 M) or CpG + Ropinirole (R, 10^–6 M) measured via flow cytometry; normalized to CpG control; n = 7 per group. Wilcoxon test was used for paired data comparisons including unstimulated vs. stimulated samples as well as PBMCs vs. B cells depleted; *p ≤ 0.05