Fig. 3
From: ERβ mediates sex-specific protection in the App-NL-G-F mouse model of Alzheimer’s disease
ERβ activation does not alter APP processing. Western blot analysis of full-length APP (FL-APP), β-CTF and α-CTF, Aβ peptide, and β-actin in A cortex and B hippocampus of female and male AppNL−G−F mice after vehicle (V) or LY treatment, as well as after sham surgery or ovariectomy (OVX in females). Quantification of C FL-APP relative to β-actin, D β-CTF relative to FL-APP, E β-CTF relative to actin, and F Aβ relative to FL-APP in male (left), and female (right), cortex (Ctx) (top), and hippocampus (bottom) (n = 3–4). * P < 0.05, ** P < 0.01, *** P < 0.001. Statistical significance was determined using unpaired t-test for males and 2-way ANOVA for females followed by uncorrected Fisher’s LSD test for multiple comparisons. Overall significant main effects of treatment or OVX are indicated